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phusion gc buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs phusion gc buffer
    Phusion Gc Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gc+buffer/Phusion+GC+Buffer+Pack/pmc13655600-25-39-54
    Average 93 stars, based on 323 article reviews
    phusion gc buffer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Impact of Helicobacter pylori infection on gut and intratumoral microbiome and its association with immunotherapy response in gastrointestinal cancer.
    Article Snippet: .. Specific primers with Barcodes, Phusion® High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs, and high-fidelity enzymes ensure efficient and accurate amplification. ..

    Article Title: Microglia-mediated protection against Alzheimer's disease pathology and detrimental effects in white matter revealed by Ptpn6 deletion.
    Article Snippet: In brief Etxeberria et al. report contextdependent microglial states through perturbation of Ptpn6, a crucial macrophage survival regulator from genome-wide screening.. Ptpn6 deletion from microglia protects neurons around plaques but can also dose-dependently trigger severe white-matter damage.. This discovery highlights the delicate balance required to harness the innate immune system for neuroprotection.

    Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications
    Article Snippet: All primers were designed using the Primer QuestTM Tool ( https://www.idtdna.com/pages/tools/primerquest , accessed on 9 May 2023) ( ). .. PCR amplification was carried out using Phusion ® High-Fidelity PCR Master Mix with GC Buffer (M0532S; New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Potential host-gut microbiota-metabolite associations in cardiorenal syndrome progression: a multi-omics integrative analysis.
    Article Snippet: .. PCR was performed in a 15 μL reaction system containing approximately 10 ng of template DNA, 2 μM of each primer, and Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs). ..

    Article Title: Soil Fertility Differences and Bacterial and Fungal Community Variation Among Subtropical Forest Stands Dominated by Different Tree Species
    Article Snippet: .. PCR amplification was performed using barcode-tagged primers and Phusion HighFidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, MA, USA). .. PCR products were checked by agarose gel electrophoresis, and qualified amplicons were purified using a Qiagen Gel Extraction Kit (Qiagen, Hilden, Germany).

    Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications.
    Article Snippet: All primers were designed using the Primer QuestTM Tool (https://www.idtdna.com/ pages/tools/primerquest, accessed on 9 May 2023) (Table S1 and Figure S4). .. PCR amplification was carried out using Phusion® High-Fidelity PCR Master Mix with GC Buffer (M0532S; New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Distinct actions of the humid heat environment on host gut microbiota, intestinal mucosal immunity, neuroendocrinology in influenza A virus-infected mouse
    Article Snippet: .. The hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified using Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs). ..

    Article Title: Structural characteristics of Ficus carica L. dietary fibers and their in vitro fermentation properties in modulating gut microbiota and metabolites
    Article Snippet: .. The V4 region of 16S rRNA (forward primer: 515F, reverse primer: 806R) was amplified by PCR using Barcode-specific primers, Phusion High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs, and high-efficiency and high-fidelity enzymes. .. 16S rRNA gene amplicon sequencing was performed on an Illumina NovaSeq 6000 platform.

    Amplification:

    Article Title: Impact of Helicobacter pylori infection on gut and intratumoral microbiome and its association with immunotherapy response in gastrointestinal cancer.
    Article Snippet: .. Specific primers with Barcodes, Phusion® High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs, and high-fidelity enzymes ensure efficient and accurate amplification. ..

    Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications
    Article Snippet: All primers were designed using the Primer QuestTM Tool ( https://www.idtdna.com/pages/tools/primerquest , accessed on 9 May 2023) ( ). .. PCR amplification was carried out using Phusion ® High-Fidelity PCR Master Mix with GC Buffer (M0532S; New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Soil Fertility Differences and Bacterial and Fungal Community Variation Among Subtropical Forest Stands Dominated by Different Tree Species
    Article Snippet: .. PCR amplification was performed using barcode-tagged primers and Phusion HighFidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, MA, USA). .. PCR products were checked by agarose gel electrophoresis, and qualified amplicons were purified using a Qiagen Gel Extraction Kit (Qiagen, Hilden, Germany).

    Article Title: Comparative Analysis of the EF-1α Intergenic Region in Babesia divergens Isolates: Insights into TA Repeat Variation and Potential Regulatory Implications.
    Article Snippet: All primers were designed using the Primer QuestTM Tool (https://www.idtdna.com/ pages/tools/primerquest, accessed on 9 May 2023) (Table S1 and Figure S4). .. PCR amplification was carried out using Phusion® High-Fidelity PCR Master Mix with GC Buffer (M0532S; New England Biolabs, Ipswich, MA, USA). ..

    Article Title: Distinct actions of the humid heat environment on host gut microbiota, intestinal mucosal immunity, neuroendocrinology in influenza A virus-infected mouse
    Article Snippet: .. The hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified using Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs). ..

    Article Title: Structural characteristics of Ficus carica L. dietary fibers and their in vitro fermentation properties in modulating gut microbiota and metabolites
    Article Snippet: .. The V4 region of 16S rRNA (forward primer: 515F, reverse primer: 806R) was amplified by PCR using Barcode-specific primers, Phusion High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs, and high-efficiency and high-fidelity enzymes. .. 16S rRNA gene amplicon sequencing was performed on an Illumina NovaSeq 6000 platform.

    Gas Chromatography:

    Article Title: Soil Fertility Differences and Bacterial and Fungal Community Variation Among Subtropical Forest Stands Dominated by Different Tree Species
    Article Snippet: .. PCR amplification was performed using barcode-tagged primers and Phusion HighFidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, MA, USA). .. PCR products were checked by agarose gel electrophoresis, and qualified amplicons were purified using a Qiagen Gel Extraction Kit (Qiagen, Hilden, Germany).

    Article Title: Structural characteristics of Ficus carica L. dietary fibers and their in vitro fermentation properties in modulating gut microbiota and metabolites
    Article Snippet: .. The V4 region of 16S rRNA (forward primer: 515F, reverse primer: 806R) was amplified by PCR using Barcode-specific primers, Phusion High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs, and high-efficiency and high-fidelity enzymes. .. 16S rRNA gene amplicon sequencing was performed on an Illumina NovaSeq 6000 platform.



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